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. 2010 Oct 1;21(19):3396–3408. doi: 10.1091/mbc.E10-06-0512

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 5. — Vps4^ΔMIT,E233Q colocalization with Did2 is Vps4-dependent. (A) In yeast lacking Vps4 (vps4Δ), Cherry-Did2 exhibits colocalization with the core ESCRT-III subunit Vps24-GFP. Dashed white lines represent the borders of individual yeast cells. Bar, 15 μm. (B) Vps4^E233Q-GFP exhibits punctate distribution and colocalizes extensively with Cherry-Did2 in vps4Δ vta1Δ cells. (C) GFP-Vps4^ΔMIT,E233Q exhibits a diffuse distribution and little colocalization with Cherry-Did2 in vps4Δ vta1Δ cells. (D) In the presence of Vps4 (vta1Δ cells), GFP-Vps4^ΔMIT,E233Q displays some punctate association that colocalizes with Cherry-Did2 (indicated by arrows). Additional micrographs demonstrating colocalization of GFP-Vps4^ΔMIT,E233Q with Cherry-Did2 in the presence of wild-type Vps4 are presented in Supplemental Figure 4.