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. 2010 Oct 1;21(19):3409–3420. doi: 10.1091/mbc.E10-03-0230

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2. — Dynamics of the blebs and intranuclear structures in transiently transfected NIH-3T3 cells. The figure was assembled from selected time points of Supplemental Movie S1, showing a NIH-3T3 cell transiently transfected with GFP-Kuk-Δ353-404.The nuclear membrane blebs formed upon GFP-Kuk-Δ353-404 expression show a very dynamic behavior. Arrows indicate examples of blebs growing and disappearing. (GFP-Kuk and GFP-Kuk-Δ353-404 showed comparable effect on nuclear morphology. Due to the more frequent appearance of blebs in GFP-Kuk-Δ353-404–transfected cells the construct was chosen for the time-lapse experiments shown.) Bar, 7 μm.