Skip to main content
. 2010 Oct 1;21(19):3409–3420. doi: 10.1091/mbc.E10-03-0230

Figure 3.

Figure 3.

Constructs used in the study. The structure of Kuk and lamin Dm0 constructs used in the study is schematically shown. The putative coiled-coil motif of Kuk (C.C., aa 137–184) is shown in white. The two highly conserved aa sequences 353–404 and 453–473 are shown in blue and the NLS in black. In Kuk-cc+ΔN185 the coiled coil of the GCN4 leucine zipper protein that has substituted the putative coiled coil of Kuk is shown in green. In the lamin Dm0 constructs, the NLS is shown in red, the rod domain in white, and the globular C-terminal domain in yellow. In LaminDm0ΔN, the N-terminal part consisting of aa 1–430 including the rod domain has been deleted. GFP-NLS-C-term contains the NLS of lamin Dm0 fused to the very C-terminal part of the protein (aa 570–622). GFP-NLS-CaaX consists of GFP fused to the NLS of lamin Dm0 followed by the CaaX motif of lamin Dm0.