Table 2.

Summary of the results from the liposome deformation experiments

Recombinant protein+farn.	Deformation of liposomes
	0.1× protein (∼0.1–0.4 μM)	1× protein (∼1–4 μM)	10× protein (∼10–40 μM)
zz-Kuk	−	+	NA
zz-Kuk-ΔN185	−	+	NA
zz-Kuk-ΔN437	−	+	NA
GFP-LaminΔN	+	+	+++
GFP-NLS-Cterm	−	−	+
GFP-NLS-CaaX	−	−	+
GFP	No effect and no colocalization with liposomes	No effect and no colocalization with liposomes	No effect and no colocalization with liposomes

The table summarizes the results of the liposome deformation experiments (see Figure 8 and Supplemental Figure S4). The effect of the respective constructs on the liposome morphology is shown by +++ (very strong), + (strong), or − (no effect). The proteins were used in 0.1×, 1×, or 10× amounts, except for the three Kuk constructs for which a 10× amount was not possible (NA). All the results shown in this table refer to the sample where the components of the farnesylation reaction were added to the liposome–protein mix. The second column shows the micromolar concentration for each of the proteins in the mixture of protein–liposomes (final volume, 20 μl), where 1× protein amount was used. The 1× protein amount refers to the amount shown on the Coomassie Blue-stained gel in Supplemental Figure S4.