Table 1.
Yeast strains used in this study
| Strain | Genotypea | Reference |
|---|---|---|
| CRY1 | MATa ade2-1 can1-100 his3-15,112 trp1-1 ura3-1 | Brickner and Fuller (1997) |
| CRY2 | MATα ade2-1 can1-100 his3-15,112 trp1-1 ura3-1 | Brickner and Fuller (1997) |
| JBY397 | SEC63-13myc:Kanr INO1:LacO128 HIS3:LacI-GFP | Brickner and Walter (2004) |
| DBY32 | SEC63-13myc:Kanr GAL1:LacO128 HIS3:LacI-GFP | Brickner et al. (2007) |
| DBY247 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY178 | clb2Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY180 | clb5Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY182 | cln3Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY184 | sic1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY243 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM1 | This study |
| DBY245 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM2 | This study |
| DBY248 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1 | This study |
| DBY249 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:nup1-ΔP1 | This study |
| DBY250 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:nup1-ΔP2 | This study |
| DBY251 | nup1Δ::Kanr INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1 P1ΔP2 | This study |
| DBY252 | SEC63-13myc:Kanr INO1:LacO128 HIS3:LacI-GFP LEU2:NUP1-PM1 | This study |
| DBY253 | SEC63-13myc:Kanr INO1:LacO128 HIS3:LacI-GFP LEU2:NUP1-PM2 | This study |
| DBY263 | cdc6-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY264 | cdc20-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY265 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1 | This study |
| DBY266 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:nup1-ΔP1 | This study |
| DBY267 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:nup1-ΔP1ΔP2 | This study |
| DBY268 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM1 | This study |
| DBY269 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM2 | This study |
| DBY270 | SEC63-13myc:Kanr GAL1:LacO128 HIS3:LacI-GFP LEU2:NUP1-PM1 | This study |
| DBY271 | SEC63-13myc:Kanr GAL1:LacO128 HIS3:LacI-GFP LEU2:NUP1-PM2 | This study |
| DBY273 | cdc28-1 INO1:LacO128 LEU2:LacI-GFP TRP1:Sec63myc | This study |
| DBY274 | cdc28-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc | This study |
| DBY279 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM1 | This study |
| DBY280 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM2 | This study |
| DBY283 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:CDC28 | This study |
| DBY284 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:CDC28 | This study |
| DBY285 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM1 | This study |
| DBY286 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM2 | This study |
| DBY293 | nup1Δ::Kanr GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:nup1-ΔP2 | This study |
| DBY295 | cdc28-1 INO1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:CDC28 | This study |
| DBY296 | cdc28-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:CDC28 | This study |
| DBY299 | cdc28-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1hyphen]PM2 | This study |
| DBY300 | cdc28-1 GAL1:LacO128 HIS3:LacI-GFP TRP1:Sec63myc LEU2:NUP1-PM1 | This study |
a All strains are isogenic with CRY1, except as indicated.