Table 2.
Primers used in this study
| Name | Sequence (5′ to 3′) |
|---|---|
| CDC28F | CCCTTACTGGGCGCACGCAGTGTATC |
| CDC28R | GAAATAAAAACAATACCCGTCTCGGTTATTC |
| CLB2confirm | GCCAATTTAGCTAGACTTTTGAAAAG |
| CLB2KOF1 | AGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAGCGGATCCCCGGGTTAATTAA |
| CLB2KOR1 | TTTATCGATTATCGTTTTAGATATTTTAAGCATCTGCCCCTCTTCGAATTCGAGCTCGTTTAAAC |
| CLB5confirm | CTTGTCATACTGTGGGTATCATATTCTG |
| CLB5KOF1 | GCGCTTTTCCCTGTATTTAAAGCCGCTGAACACCTTTACTGAACACGGATCCCCGGGTTAATTAA |
| CLB5KOR1 | AAAATGTAAAGAGTATGCGAATTCATGAGCATTACTAGTACTAATGAATTCGAGCTCGTTTAAAC |
| CLN3confirm | GGCCTTGGGTTTTTGCCCTCATC |
| CLN3KNOF1 | CTTTTACTCTCGTTCAAGACACTGATTTGATACGCTTTCTGTACGCGGATCCCCGGGTTAATTAA |
| CLN3KOR1 | AAATTTTAATTTATTTGTTGTTAAATGCATTTTTTTTTTGTCGTTGAATTCGAGCTCGTTTAAAC |
| KanB | CTGCAGCGAGGAGCCGTAAT |
| NUP1F | GACACGAAGTCTCTCTGGAGTGCTTTG |
| NUP1R | CATCATTGTTGTGAATACGCACCCTAC |
| NUP1ΔP1F | CATCTACTCCTTGCCCTATTAAAAAC |
| NUP1ΔP1R | GTTTTTAATAGGGCAAGGAGTAGATG |
| NUP1ΔP2F | CAAGGACAATGAGTGTCCATCTAAGAAAACTTC |
| NUP1ΔP2R | GAAGTTTTCTTAGATGGACACTCATTGTCCTTG |
| NUP1PM1F | CATCTACTCCTGACCCTATTAAAAAC |
| NUP1PM1R | GTTTTTAATAGGGTCAGGAGTAGATG |
| NUP1PM2F | CAAGGACAATGAGGATCCATCTAAGAAAACTTC |
| NUP1PM2R | GAAGTTTTCTTAGATGGATCCTCATTGTCCTTG |
| SIC1confirm | GTGAGACCAGAGTTGCAGAGATGGAC |
| SIC1KOF1 | CCTCTACGGAATTTTGACCCTTGAAGCAGGGACTATTACACGAAACGGATCCCCGGGTTAATTAA |
| SIC1KOR1 | GTAAATAAAATATAATCGTTCCAGAAACTTTTTTTTTTCATTTCTGAATTCGAGCTCGTTTAAAC |
| STE2confirm | GCATAGAACGAACTGTAGAATAGTCCGG |
| STE2KOF1 | GTTACTTAAAAATGCACCGTTAAGAACCATATCCAAGAATCAAAACGGATCCCCGGGTTAATTAA |
| STE2KOR1 | ATACCGAAGGTCACGAAATTACTTTTTCAAAGCCGTAAATTTTGAGAATTCGAGCTCGTTTAAAC |