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. 2010 Oct 1;21(19):3433–3442. doi: 10.1091/mbc.E10-04-0347

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution-Noncommercial-Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 6. — Heat-shocked transgenic larvae demonstrate increased nuclear localization of AMPKα in vivo. Live animal images of 3rd instar Drosophila larvae expressing GFP-tagged (A and D) or mCherry-tagged wild-type AMPKα (B and E), mCherry-tagged truncated AMPKαΔC (C), or GFP-tagged APC2 (containing both NLS and NES sequences). Animals in D–F were subjected to heat shock at 37°C for 1 h, followed by a 15-min recovery at 25°C. All transgenic proteins are expressed using the Gal4-UAS system, driven by Ubiquitin-Gal4. Bar, 20 μm.