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. 2010 Sep 29;5(9):e13064. doi: 10.1371/journal.pone.0013064

Figure 2. N-terminal regions of DAAM1 are involved with actin stress fibers and centrosome targeting.

Figure 2

(A) GFP-Flag-DAAM1 (1–440) was expressed in HeLa and analyzed by confocal imaging. DAAM1 was immuno-localized with anti-GFP (in green). Cells were counter-stained with TRITC-phalloidin, anti-myosin IIA or anti-myosin IIB (red). (B) Table summarizing the localization of various DAAM1 constructs tested in this study. (C) COS-7 cells expressing DAAM1(100–350) form large filaments enriched for myosin IIB that do not colocalize with F-actin. Treatment with various acto-myosin disrupting drugs with the indicated concentration for 45 min do not abolish the presence of these fibrils. Bars  = 10 µm.