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. 2010 Sep 29;5(9):e13069. doi: 10.1371/journal.pone.0013069

Figure 1. Effect of hfq deletion on pigmentation.

Figure 1

S. aureus strains WT, Δhfq-8325 and rs-hfq-8325 were cultured for 12 h. The pigments of different strains were determined. The carotenoid pigment of Δhfq-8325 was increased (A) and showed the characteristic triple peak spectral profile of carotenoid (B). However, the color of rs-hfq-8325 was similar to the wild type. The expression level of crtM was detected by qRT-PCR. The results of qRT-PCR showed that the expression of crtM was increased in Δhfq-8325 and recovered in rs-hfq-8325 (C). The results shown were representative of three independent experiments (* P<0.01). WT, S. aureus 8325-4; Δhfq-8325, 8325-4 with an hfq::kan mutation; rs-hfq-8325, the restoration of Hfq activity in Δhfq-8325.