Table 1. Sequence throughput obtained with the three UHTS platforms analyzed.
Sequencing technology | Reads | Count | Discarded reads | Average length of a read (nt) | Bases | Trimmed bases |
Roche 454 (1/8) | Total (raw) | 99,317 | 318 | 31,615,489 | ||
After trimming | 99,317 | 0.00% | 232 | 23,010,105 | 27.2% | |
After trimming (>20 nt) | 98,975 | 0.34% | 232 | 23,005,448 | 27.2% | |
Illumina GA (1 lane) | Total (raw) | 4,611,113 | 34 | 156,777,842 | ||
After trimming | 4,610,388 | 0.02% | 30 | 137,405,021 | 12.4% | |
After trimming (>20 nt) | 4,245,639 | 7.9% | 31 | 132,052,133 | 15.8% | |
ABI SOLiD (1 quad) | Total (raw) | 17,287,756 | 50 | 862,377,074 | ||
After trimming | 17,287,610 | 0.00% | 31 | 534,615,489 | 38.0% | |
After trimming (>201 nt) | 13,597,456 | 21.3% | 36 | 487,053,627 | 43.5% |