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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Hepatology. 2010 Oct;52(4):1443–1454. doi: 10.1002/hep.23829

Figure 7. Conditions yielding mature Cholangiocytes.

Figure 7

Formation of bile duct structures within 7-10 days requires that the hHpSCs be cultured in MKM-C (MKM supplemented with VEGF, HGF) and plated onto mature stellate cells (pericytes) or myofibroblasts (A). The feeders can be replaced by embedding the cells into a mix of 60% type I collagen gel and 40% hydrogel (Hyaluronans or Matrigel) and in the HDM tailored for cholangiocytes (B-E). The cells form ramifying ducts and tubes that express cytokeratin 19 (green) and secretin receptor (red) (E) as well as other markers (e.g. aquaporins, ABAT, data not shown) for cholangiocytes. The image in B is a composite of multiple images to show a large colony within the hydrogel/matrix mixture. The images in C and D are enlargements of some of the ducts from the colony in B.