Figure 1. Myeloid CD11b⁺Gr1⁺ cells accumulate in Tgfb1^−/− livers.

(A) Abundant myeloid cells (arrows) are observed in liver from day 11 Tgfb1^−/− mice (−/−) but not age-matched Tgfb1^+/− mice (+/−). Bar = 50 μm. (B, C) CD4⁺ T cells and Gr1⁺ cells were counted after isolation from livers from Tgfb1^−/− mice and Tgfb1^+/− mice at 4, 7, and 11 days of age. n = 3 or more mice per group. (D) Isolated liver Gr1⁺ cells from Tgfb1^−/− mice and Tgfb1^+/− mice co-express CD11b. Solid: Isotype control. (E) After cytospin, Tgfb1^−/− liver CD11b⁺ cells were stained with Wright-Giemsa. ** p < 0.01; *** p < 0.0001.