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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Pharmacol Ther. 2010 Aug 21;128(2):375–384. doi: 10.1016/j.pharmthera.2010.08.001

Fig. 3.

Fig. 3

Role of Beclin-1 and ATG5 in Ad.mda-7-induced apoptosis. DU-145 cells were transfected with the indicated siRNAs, and cell viability was determined by MTT assay (A), Caspase-Glo 3/7 assay for caspase-3 expression (B), and colony formation assays (C) 48 hours after infection with Ad.mda-7 (10, 50, or 100 pfu/cell) or Ad.vec (100 pfu/cell). C, colony formation assays in monolayer culture. Colonies were fixed, stained, and counted (>50 cells) 2 weeks after plating. D, Expression profile of Beclin-1 and ATG5 at the mRNA level 48 h after Ad.mda-7 infection (50 or 100 pfu/cell) of DU-145 cells. ★, P < 0.05; ★★, P < 0.001, compared with control. Data reproduced with permission from Bhutia et al. Mechanism of autophagy to apoptosis switch triggered in prostate cancer cells by antitumor cytokine melanoma differentiation associated gene 7/interleukin-24. Cancer Res. 70 (2010), 3667–3676.