Figure 4.

Effects of S3 and pS3 peptides on TEA-induced GluR1 addition. (a) Representative images showing SEP-GluR1 fluorescence on dendritic regions before and after cLTP induction by 10 min TEA treatment. The neurons were treated with different peptides for 6 hr prior to cLTP treatment. Regions enclosed by the box are shown at a higher magnification. Arrows indicate sites of new SEP-GluR1 addition. Scale bar = 5 μm. (b) Quantification showing the changes in the number of bright SEP-GluR1 puncta after 10 min TEA or HBS treatment. Asterisks: p<0.01 comparing to the HBS group by Student's t-test. The TEA+S3 group is statistically different from the TEA group (p<0.01). Numbers indicate the number of cells examined in each group. (c) The box-whisker plot showing the changes of SEP-GluR1 fluorescence (ΔF/F) of all individual spines after 10 min TEA or HBS treatment. The bottom and top of the box represent the 25^th and 75^th percentile and the line near the middle of the box depicts the median (50%). The ends of the whiskers represent the 15^th and 85^th percentiles, respectively. Standalone asterisk: p<0.01 comparing to the HBS control (t-test). The asterisk for compared TEA and TEA+S3 groups: p<0.01 (t-test). (d) Quantification showing the baseline density of spine number and SEP-GluR1 puncta number before and after 6 hr peptide treatment. Asterisk: p<0.05 compared to the group without peptide treatment (Student's t-test). (e) Analysis of the spine head/neck ratio after 6 hr peptide treatment. Error bars in (b, d, e) represent s.e.m.