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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Nat Neurosci. 2010 Sep 5;13(10):1190–1198. doi: 10.1038/nn.2623

Figure 3.

Figure 3

Continued Pet-1 function is required for 5-HT neuron firing properties and inhibitory autoreceptor function. (a, b) Whole cell current clamp recordings measuring spontaneous firing of Yfp+ neurons with indicated genotypes (c) Quantification of firing frequencies in (a, b), +/+, n=12; Pet-1eCKO, n=19; * p<0.05, two tailed t-test. (d, e) Whole cell voltage clamp recordings measuring current changes of Yfp+ neurons induced with either 1 or 10 μM of 5-HT1A agonist 8-OH-DPAT. A ramp voltage was applied at 200 mV/s. The intersection voltage, -87 mV, of the control and 8-OH-DPAT trace in (d) was close to the estimated K+ equilibrium potential (-99 mV), considering that recordings were not corrected for the liquid junction potential of around 10 mV. (f) Quantification of current changes at -110 mV in (d, e); * p<0.05, *** P<0.001; two tailed t-test (g–j) In situ hybridization of 5-HT1A (g, h) and 5-HT1B (i, j) in control and Pet-1eCKO mice. Scale bar is 200 μm. Error bar is mean ± s.e.m.