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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Lab Invest. 2010 Jun 28;90(10):1415–1424. doi: 10.1038/labinvest.2010.117

Figure 5. ErbB4 regulates COX-2 mRNA expression.

Figure 5

(A) Cells were exposed to HRG or TNF in the presence of Actinomycin D (ActD; 10 μg/ml) and COX-2 protein levels were determined by Western blot analysis. (B) JM-b/CYT-2 expressing cells (YAMC-ErbB4) were exposed to tumor necrosis factor (TNF, 100 ng/ml) or HRG (100 ng/ml) for 3h; COX-2 mRNA levels were determined by RT-qPCR. (C) YAMC cells were transfected with non-targeting or ErbB4-specific siRNA pools for 72h. Cells were stimulated with TNF and COX-2 levels determined by RT-qPCR. (D) Cells were exposed to Actinomycin D for indicated times and COX-2 mRNA levels were determined by RT-qPCR. (E) Expression of RBM3, HuR, and CUGBP-2 protein in YAMC-Vec and YAMC-ErbB4 cells was determined by Western blot analysis.