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. Author manuscript; available in PMC: 2011 Oct 5.
Published in final edited form as: Langmuir. 2010 Oct 5;26(19):15430–15435. doi: 10.1021/la1021824

Figure 4.

Figure 4

Time-resolved response from human α-thrombin binding to microrings modified with anti-(human thrombin) antibody at pH 7.4 in the presence (a) and absence (b) of 100 mM aniline. The microrings are initially in BSA-PBS buffer and thrombin is introduced at t = 5 min. Thrombin is then introduced and incubated for 10 min, after which BSA-PBS buffer is flowed to observe antigen dissociation. The remaining antigen-antibody interactions on the surface are then disrupted via exposure to a low pH (pH 2.2) glycine buffer for 2 min (not shown) to regenerate the sensor for subsequent detection experiments.