Fig. 5.

Effect of P2X7R antagonists on human osteoclast resorption. Osteoclasts were grown on dentine discs from blood monocytes in the presence of recombinant M-CSF and RANKL. Antagonists were introduced throughout the culture by inclusion in the medium at each medium change at the concentrations shown on the x axis. Vehicle was complete α-MEM plus recombinant RANKL (30 ng/ml) and M-CSF (25 ng/ml) and 0.1% DMSO for KN62, AZ15d and AZ408. After 3 weeks in culture, discs were fixed and stained with toluidine blue or TRAP. The area of resorption excavated by these cells was determined by point counting [26]. Data show means ± SEM, *p < 0.05, **p < 0.01 ***p < 0.0001. Graphs representative of four repeat experiments, n = 7. a oATP, b KN62, c AZ15d, d A-438079 and e AZ408