Fig. 5.

Microfluorometric recordings of [Ca²⁺]_i from fura-2-loaded Vero and GH₄ cells exposed to NheA + B + C (NVH75/95), NheA + B (MHI1672) and NheB + C (MHI1761). The ratio between emissions at the excitation wavelengths 360 and 380 nm reflects [Ca²⁺]_i. Nhe was applied continuously by pressure ejection onto the cells from the time indicated by the arrows. NheA + B + C induced rapid elevation of [Ca²⁺]_i in both Vero and GH₄ cells (a, d). NheA + B induced elevation of [Ca²⁺]_i only in GH₄ cells (b, e), while NheB + C had no effect on [Ca²⁺]_i in either of the tested cell types (c, f)