Table 1.
K+ efflux from Vero and GH4 cells induced by B. cereus strains expressing different combinations of Nhe components
| B. cereus (toxin combination) | K+ efflux (ΔmV) | |
|---|---|---|
| Vero cells | GH4 cells | |
| NVH75/95 (NheA + B + C) | 18.0 ± 2.5* (3) | 17.0 ± 3.1* (3) |
| MHI1672 (NheA + B) | 8.6 ± 1.2* (3) | 15.0 ± 3.0* (4) |
| MHI1761 (NheB + C) | 2.6 ± 1.2 (3) | 5.3 ± 2.1 (4) |
| Dialysis buffer | 2.0 ± 0.4 (4) | 2.7 ± 1.4 (3) |
Results are expressed as change in voltage (mean ± SE) recorded by a K+-selective electrode in the bath after 10-min exposure to the B. cereus culture supernatants. The recording chamber contained 106 cells suspended in 2.5 ml choline-based bathing solution, and 50 μl of the various supernatants was added as indicated. The number of experiments is given in parentheses
* Values significantly different (P < 0.05) from the dialysis buffer control (one-way ANOVA and Holm-Sidak pairwise comparisons)