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. 2010 Oct 1;24(19):2127–2132. doi: 10.1101/gad.593410

Figure 1.

Figure 1.

The gibberellin biosynthetic gene GA3ox1 is a direct target of IND. (A) Mature wild-type Arabidopsis fruit and schematic cross-section showing the different tissues. The regulatory network controlling the development of fruit tissues is also indicated. Expression of the valve margin identity genes (IND, ALC, and SHP1/2) is restricted to the valve margins by the FUL and RPL activities in the valve and the replum, respectively. Bar, 1 mm. (B) Transcript profiling assay using 7-d-old 35S∷IND:GR seedlings before (−DEX) and after (+DEX) 6 h of DEX treatment, showing an increase in GA3ox1 mRNA accumulation in response to DEX treatment compared with UBQ10 mRNA as internal control (n = 3). Error bars represent standard deviation (SD). (C) ChIP showing the direct interaction of IND-GR with the GA3ox1 gene. DNA obtained from pull-down with the GR antibody has been analyzed by qPCR using specific primers for the GA3ox1 gene. Values correspond to the ratios between pull-down and input DNA, both initially normalized to the Mu-like transposon. Values for four biological repeats are represented. P-value, <0.01. (D) β-Glucuronidase expression of AtGA3ox1 TC-GUS in young fruit (stage 15 according to Smyth et al. 1990) in the wild type (WT) and ind-1 mutant (ind-1). Bar, 500 μm. (E) β-Glucuronidase expression of AtGA3ox1 TC-GUS in young fruit (stage 15) cross-sections in th wild type and ind-1 mutant (ind-1). Bar, 100 μm.