Figure 3.
Low GA levels cause SL defects that resemble those of the alc mutant. (A) Diagram (based on a mPS-PI/confocal image) of the different cell layers in the wild-type valve margins: SL (orange) and LL (red). (B,C) TEM images showing valve margin tissues (cross-section) of the wild type (WT) (B) and ga4-1 mutant (C). SL cells are marked with asterisks (*). (R) Replum; (V) valve. Bar, 10 μm. (D) Quantification of cell sizes in the cell layer corresponding to the SL in the wild type (n = 38), alc-1 (n = 46), ga4-1 (n = 41), pIND >> GA2ox (n = 34), and alc-1; ga4-1 double mutant (n = 49). Error bars represent standard deviation (SD).