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. 2010 Oct 1;24(19):2127–2132. doi: 10.1101/gad.593410

Figure 4.

Figure 4.

DELLA proteins interact with ALC and inhibit valve margin development in the absence of GA. (A) Yeast two-hybrid interaction using ALC as a bait and GAI, RGA, and RGL2 as prey. (Top and bottom panels) Dilution series plated on selective medium (−Leu Trp His Ade) and control medium (−Leu Trp), respectively. (B) Projections of confocal sections showing expression of pRGA∷GFP-RGA in medial tissues of stage 15 gynoecium: untreated (Ø), treated with 1 μM PAC (+PAC), or treated with 10 μM gibberellin (+GA3) for 24 h. (C) SEM images showing the base of a stage 17b fruit of the ga1-3 mutant treated with 1 μM GA3 (left panel) or 100 μM GA3 (right panel), and of the ga-3; rga-t2; gai-t6 triple mutant (middle panel). Bar = 100 μm. (D) Model of DELLA-mediated control of ALC by IND. IND induces GA biosynthesis via the direct activation of GA3ox1. Without GAs, ALC interacts with DELLA proteins. During valve margin development, IND-induced local GA production leads to DELLA degradation and release of ALC, which then controls SL genes.