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. Author manuscript; available in PMC: 2011 Mar 9.
Published in final edited form as: N Engl J Med. 2010 Sep 9;363(11):1005–1015. doi: 10.1056/NEJMoa0907847

Figure 2. Assay Procedure for the MTB/RIF Test.

Figure 2

Two volumes of sample treatment reagent are added to each volume of sputum. The mixture is shaken, incubated at room temperature for 15 minutes, and shaken again. Next, a sample of 2 to 3 ml is transferred to the test cartridge, which is then loaded into the instrument. All subsequent steps occur automatically. The user is provided with a printable test result, such as “MTB detected; RIF resistance not detected.” PCR denotes polymerase chain reaction.