Histological analysis of control and class I and class II mutant hearts. A and B, Cre+ control. C and D, Cre− control. E and F, Class I mutant. G and H, Class II mutant. B, D, F, and H are high-magnification pictures of the boxed areas in A, C, E, and G, respectively. Hearts were sectioned and stained with Masson's trichrome, and fibrous tissue appears in blue. In all control as well as class I and class II mutants, the AV node appeared compact and ovoid. Ao indicates aorta; AVN, AV node; His, His bundle; LV, left ventricle; and RV, right ventricle. Scale bar 500 μm (A, C, E, and G) and 200 μm (B, D, F, and H).