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. Author manuscript; available in PMC: 2010 Sep 30.
Published in final edited form as: J Biol Chem. 2004 Nov 19;280(5):3946–3953. doi: 10.1074/jbc.M407876200

FIG. 3. A-to-I editing of 36-bp but not 29- or 19-bp siRNA.

FIG. 3

A, ADAR1p150 (wild-type and E912A mutant) and ADAR2 (wild-type and E396A mutant) were tested for binding at 30 °C for 60 min to 29- or 36-bp siRNA (10 pm), and the reaction products were analyzed by EMSA. ADAR1p150, 0.05, 0.075, 0.1, 0.15, 0.2, 0.5, 1, 5, and 10 nm. ADAR2, 1, 2, 2.5, 5, 10, 20, 50, 100, and 250 nm. B, TLC analysis of siRNAs bound by ADAR1p150. Varying sizes of siRNA complexed with ADAR1p150 (wild-type) were analyzed for A-to-I editing of internal adenosine residues.