Fig. 2.
ThT at the subunit interfaces of β2mholo. (A) A ribbon representation of the β2mholo hexamer. This is a trimer of dimers with alternate subunits drawn in blue and green. The ThT molecules are drawn in orange and can be seen intercalated at right angles to the strands of the intersheet interfaces. (B) Close up of a single, two-subunit interface with either one (Right) or the other (Left) of the subunits drawn as a surface while the other is shown as sticks. Two subunits from the ThT-free crystal structure of this hexamer are shown as white sticks (PDB ID: 3CIQ). Note, alignment of the two subunits was deliberately performed using only backbone atoms of the left (green) subunit. This results in the left panel showing only side-chain rotamer changes while the right panel captures all of the subunit–subunit displacements.