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. 2010 Sep 13;107(39):16934–16939. doi: 10.1073/pnas.1002894107

Fig. 3.

Fig. 3.

Incubation of resting CD4+ T cells with CCL19 increases HIV-1 nuclear localization and integration. Resting CD4+ T cells were cultured for 3 d with media alone (unactivated) or with PHA/IL-2 or with the chemokine CCL19, and infected with HIV NL4.3. Quantitative PCR for (A) early reverse transcripts (strong stop DNA), (B) full-length DNA, (C) the nuclear products 2-LTR circles, and (D) HIV-1 integrated DNA was performed and quantified as copies/million cells. The efficiency of each step in reverse transcription, nuclear localization, and viral integration is shown in parentheses below each graph. The efficiency of each step was calculated as described in Materials and Methods. Replicates (A and B) (red open circles) and the mean (open column) from one experiment are shown. Results from one of two experiments are shown. Infection with heat-inactivated virus as control for background strong stop and full-length DNA is shown (black circles). (E) Kinetics of entry and reverse transcription following incubation with PHA/IL-2 or CCL19 or in unactivated cells. Strong stop DNA, full-length DNA, and 2-LTR circles were quantified 3, 6, 12, 24, and 48 h postinfection and integrated DNA was quantified at 96 h. Representative results from one of two experiments are shown.