Table 1. Effect of Condition Changes on [3H]-FLC Import in SC5314.
| Row | Conditions | Condition details | Importa | Relative changeb |
| 1 | Killed Cells | Methanol | 289±57 | 0.12 |
| Heat | 470±45 | 0.20 | ||
| 2 | Temperature | 4°C | 556±54 | 0.24 |
| 25°C (WT) | 2335±193 | 1.00 | ||
| 30°C | 2845±130 | 1.22 | ||
| 37°C | 2583±417 | 1.11 | ||
| 3 | Growth | OD600 0.4 (log) | 23,394±1795 | 4.67 |
| OD600 8–10 (post diauxic shift)(WT) | 5007±656 | 1.00 | ||
| 4 | Morphology | Germ tubes (Hyphae) | 8826±462 | 3.78 |
| Yeast (WT) | 2336±193 | 1.00 | ||
| 5 | pH | pH 3 | 2754±96 | 1.00 |
| pH 5 (WT) | 2766±164 | 1.00 | ||
| pH 7 | 2588±187 | 0.94 | ||
| pH 9 | 2299±339 | 0.83 | ||
| 6 | Low Oxygen | Growth in micro-aerophilic conditions | 11946±466 | 4.55 |
| Growth under normal conditions | 2624±174 | 1.00 |
a
Import was analyzed as described in the Materials and Methods. Values are CPM/1×108 cells ± SE. Values are representative of three independent experiments. Conditions were analyzed independent of other conditions.
b
Values are relative to import of live SC5314 (WT) under standard conditions at 24 h.