Figure 4. Electron microscopic images of DI-T and VSV particles in clathrin-coated pits.

(A) Electron micrographs depicting DI-T particles at early (left) or late (right) stages of clathrin-dependent endocytosis. BSC1 cells were incubated with ∼1000 particles per cell for 10 min. at 37°C. Cells were then fixed, and samples were processed for ultra thin sectioning and viewed as described in the materials and methods. (B) Electron micrographs of VSV particles at sequential (left to right, top to bottom) stages of clathrin-dependent endocytosis. Vero cells were inoculated with VSV at an MOI of 5, and samples were prepared for analysis at 6 h post-infection.