. 2010 Sep 30;6(9):e1001127. doi: 10.1371/journal.ppat.1001127

Table 1. Summary of kinetic and fluorescence intensity data.

Particle(s)	Fluorescent protein(s)	Treatment	# Expts/# Cells	Events analyzed	# Events	Lifetime	% Lifetime	% Max fluorescence
DI-T	σ2		1/3	DI-T	62	47+/−13 s	ND	ND
DI-T+VSV	σ2		1/4	pits	212	35+/−10 s	100+/−24	100+/−23
				DI-T	36	43+/−14 s	128+/−37^**	119+/−38^**
				VSV	29	75+/−22 s	224+/−68^**	148+/−48^**
	LCa		1/3	pits	209	44+/−19 s	100+/−34	100+/−26
				DI-T	48	44+/−14 s	100+/−30	99+/−26
				VSV	34	89+/−36 s	222+/−104^**	130+/−38^**
DI-T+VSV	σ2	+latB	2/4	pits	193	47+/−14 s	100+/−27	100+/−27
		+latB		DI-T	46	54+/−21 s	118+/−46^*	133+/−42^**
		+latB		VSV entry	20	141+/−104 s	298+/−198^**	207+/−59^**
		+latB		trapped VSV	31	615+/−216 s	1283+/−430^**	192+/−73^**
DI-T	LCa + cortactin		1/3	pits	155	51+/−19 s	100+/−34	100+/−50
				DI-T	30	65+/−28 s	129+/−63^*	130+/−63^*
			1/3	pits	220	52+/−24 s	100+/−36	100+/−66
VSV	LCa + cortactin			VSV	21	166+/−63 s	335+/−105^**	300+/−164^**

Kinetic and fluorescence values are provided as the mean +/− SD for all events in a given context. The number of experiments and cells analyzed is indicated. The absolute lifetimes are expressed in seconds (s). The % lifetime and % maximum fluorescence intensity values were calculated as described in the legend of Figure 3C. The % maximum fluorescence intensity of cortactin is provided for events analyzed in cells co-expressing mCherry-LCa and cortactin-eGFP. A two-tailed Student's t-test was used to determine whether data from 2 categories differ in a statistically significantly manner.

(*) denotes a statistical difference with a p-value <0.005 and >0.00005 when comparing data in a given category to data for pits lacking virus in the same category.

(**) similarly denotes a p-value <0.00005. All values for VSV are significantly different (p<0.005 for % max AP-2 fluorescence; p<0.00005 for all other categories) from those measured for DI-T particles in the same context. ND, not determined.