Table 1. SEC-MALS analysis of mDia1 proteins and complexes.
mDia1 Proteins and Complexes | Mw Determined by MALS (kDa) | Sequence Predicted Mw for monomer (kDa) | ||
N+C | N | C | ||
N: DID-DD, residues 131–458C: FH2-DAD, residues 736–1200 | 345 | 92 | 38 | 54 |
N: GBD-DID-DD, residues 72–458C: FH2-DAD, residues 736–1200 | 354 | 98 | 44 | 54 |
N: DID-DD-CC, residues 131–477C: FH2-DAD, residues 736–1200 | 195 | 94 | 40 | 54 |
C: FH2-DAD, residues 736–1200 | 109 | - | - | 54 |
mDia1-DID-C: residues 131–1255 | 278 | 125 | - | - |