Figure 6. Effect of ESTA-1 binding on cell adhesion and cell viability.
ES-Endo were incubated with doxycycline (1000 ng/ml) for 5 hours followed by two concentrations of ESTA-1 (50 and 100 nM) for 30 minutes. sLex positive HL-60 cells were added to each well and incubated at 4 °C for 30 minutes and cell adhesion was analyzed under 100x final magnification (A). Error bars, mean ± SEM; *,P<0.05, **, P<0.01 vs. Dox+/ESTA-, Student's t test. ES-Endo were induced with doxycycline (2000 ng/ml) for 5 hours and incubated with culture media containing ESTA-1 at indicated concentrations for 48 hours (B). The cells were washed and incubated with MTT for 4 hours, and the absorbance at 570 nm was measured. The data was normalized by untreated cells (without ESTA-1) as 100%. Experiments were repeated three times.