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. 2010 Sep 30;5(9):e13117. doi: 10.1371/journal.pone.0013117

Figure 4. Intracellular STAT1-CC upregulates HLA class I surface expression in IFN-γ resistant (GR17-1) cells.

Figure 4

GR17-1 cells were transfected with STAT1, STAT1-CC or STAT1-CC-Y701F plasmid using FuGENE 6 reagent for 72 hours with or without IFN-γ treatment. After 72 hours, the cells were harvested and stained with a phycoerythrin-conjugated anti-human HLA antibody. Surface HLA-1 expression was then quantified by flow cytometry. (A) Each histogram is representative of six independent experiments. The red cell population in each histogram represents IFN-γ naïve cells and the blue cell population represents IFN-γ treated cells. (B) Mean fluorescence intensity of six independent experiments per group. Values represent fold increase in HLA1 expression after the addition of IFN-γ. Mock represents GR17-1 cells without plasmid transfection. Asterisk (*) denotes a significant (p<0.05) increase in HLA1 in STAT1-CC transfected cells plus IFN-γ compared to mock.