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The IFN-γ resistant cell line (GR17-1) was transfected with each STAT1 expression plasmid and treated with or without IFN-γ. At 24 hours post-transfection protein lysates were obtained and examined for p-PKR and p-EIF2α levels by western blot analysis. A protein lysate from the S9-13 cell line was obtained 30 minutes after IFN-γ addition and used as a positive control.
