Table 3.
Copy number of vector DNA in tissues over time following intravenous administration of 2 × 1010 genome copies of AAV.CB.Luciferase.
| Liver | AAV2/1 | AAV2/2 | AAV2/3 | AAV2/4 | AAV2/5 | AAV2/6 | AAV2/7 | AAV2/8 | AAV2/9 | 2/rh10 |
|---|---|---|---|---|---|---|---|---|---|---|
| DOL 5 | 37.101±9.391 | 18.725±2.007 | 73.741±34.883 | 2.254±1.644 | 28.004±8.597 | 4.014±1.528 | 22.722±10.777 | 56.782±25.790 | 48.977±23.577 | 292.126±95.629 |
| DOL70 | 0.031±0.006 | 0.013±0.000 | 0.035±0.004 | 0.007±0.004 | 0.014±0.006 | 0.014±0.006 | 0.012±0.003 | 0.124±0.042 | 0.114±0.044 | 1.699±0.950 |
| DOL100 | 0.012±0.006 | 0.003±0.001 | 0.005±0.001 | < 1 in 1000 | 0.009±0.003 | 0.002±0.000 | 0.014±0.007 | 0.071±0.024 | 0.095±0.032 | 0.512±0.116 |
| Muscle | AAV2/1 | AAV2/2 | AAV2/3 | AAV2/4 | AAV2/5 | AAV2/6 | AAV2/7 | AAV2/8 | AAV2/9 | 2/rh10 |
| DOL 5 | 4.542±2.770 | 1.331±0.135 | 3.403±2.314 | 2.502±0.459 | 0.542±0.257 | 0.446±0.081 | 3.832±1.253 | 0.732±0.258 | 3.148±1.461 | 19.810±4.169 |
| DOL70 | 0.050±0.007 | 0.065±0.026 | 0.041±0.003 | <1 in 1000 | 0.033±0.009 | 0.072±0.028 | 0.261±0.159 | 0.040±0.015 | 0.240±0.061 | 1.092±0.212 |
| DOL100 | 0.131±0.008 | 0.159±0.017 | 0.148±0.124 | 0.044±0.034 | 0.028±0.028 | 0.063±0.037 | 0.341±0.111 | 0.146±0.119 | 0.140±0.005 | 1.196±0.211 |
Abbreviations: AAV, adeno-associated virus; DOL, day of life.
Determined by quantitative PCR of transgene firefly luciferase, according to standards generated with pEF1α-luciferase12. The mean ± SD (n=3) are shown for each vector in each tissue.