Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 30;5(9):e13122. doi: 10.1371/journal.pone.0013122

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Syngkon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A) PCR amplification with internal primers for ctx (lane 1), hapA (lane 3), prtV (lane 5) and VC1649 (lane 7) in strain C6709 and for ctx (lane 2), hapA (lane 4), prtV (lane 6) and VC1649 (lane 8) in strain CHA6.8ΔprtVΔVC1649. The primer sequence used in the above experiment is given in Table 1. (MW) denotes molecular weight marker (20 kb-75 bp marker, Fermentas). B) Native PAGE (10%) with ammonium sulphate precipitated proteins of C6709 (lane 1), CHA6.8 (lane 2), CHA6.8ΔprtV (lane 3) and CHA6.8ΔprtVΔVC1649 (lane 4). The * shows the protein band with sequence homology to the 59-kDa serine protease (VC1649). This band is absent in strain CHA6.8ΔprtVΔVC1649 (lane 4).