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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: Nature. 2010 Aug 29;467(7315):608–611. doi: 10.1038/nature09338

Figure 3. Rai1 functions to clear mRNAs in cells subjected to glucose or amino acid starvation.

Figure 3

Wild-type (WT) or rai1Δ yeast strains were grown in complete medium at 30°C to an OD600 of 0.6 and subsequently cultured in complete medium (a), glucose minus medium (b) or amino acid minus medium (c) for 30 min followed by addition of 5 µg/ml thiolutin. Total RNAs were isolated and detected at the indicated times following thiolutin addition by Northern Blot analysis and RNA half-lives (t1/2) quantified from three independent experiments normalized relative to the 18S rRNA are presented.