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. 2010 Apr 30;95(10):1633–1641. doi: 10.3324/haematol.2010.023267

Figure 4.

Figure 4.

Ets-1 expression levels in CD34+ hematopoietic progenitor cells purified from normal and leukemic samples. (A) qRT-PCR of Ets-1 mRNA was performed on the indicated days in hematopoietic progenitor cells grown in liquid phase unilineage granulocytic culture. GAPDH endogenous control was used for normalization. Error bars represent mean ± SD (n=3). (B) Western blot of Ets-1 protein in CD34+ hematopoietic stem cell granulocytic culture with anti- Ets-1, -pS282/285, -pT38 and -actin antibodies. (C) qRT-PCR of p51Ets-1 mRNA in CD34+ cells derived from peripheral blood (PB) and cord blood (CB) of healthy donors and AML patients (AML). (D) qRT-PCR of p42Ets-1 mRNA in normal CD34+ cells and AML patients. (E) FACS analysis of cell surface antigens following ATRA treatment of leukemic blasts from an t(15;17) AML patient. (F) Western blot of Ets-1 protein in ATRA-treated APL blasts.