Figure 2.
Oncogene analyses of plasma cells and memory B cells: (A) from a CCND1-positive MM patient (MM-1) and (B) from a CCND1-positive MGUS patient (MGUS-2). Global RT-PCR products were screened for CCND1 expression using real-time PCR, with 100 sorted cells per tube in each case. CCND1-positive or negative real-time PCR products were run on a gel and bands corresponding to CCND1 were excised from the gel and sequenced. The oncogene/β-actin ratio determined by real-time PCR is shown below each positive case. P, positive control; N, negative control (H2O); lanes 1 and 2, healthy memory B cells; lanes 3 and 4, oncogene-positive memory B cells; lane 5, RT control; lanes 6 and 7, myeloma plasma cells; lanes 8 and 9, healthy plasma cells.