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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Free Radic Biol Med. 2010 Aug 6;49(9):1361–1367. doi: 10.1016/j.freeradbiomed.2010.07.016

Figure 4.

Figure 4

Wild-type and C247S mutant Txnip equivalently suppress HRE-luciferase promoter activity in MLE-12 cells. Data are expressed as mean ± SEM and are representative of 2–3 individual experiments. A) HRE-luciferase activity was measured as described in Methods in room air-exposed MLE-12 cells following transient overexpression of wild-type or C247S Txnip for 24 h and was decreased by 43% and 39%, respectively, compared to control-transfected cells (n=10–13; *p<0.001 vs control). B) HRE-luciferase activity was measured in MLE-12 cells exposed to 1% oxygen following transient overexpression of Txnip for 24 h and was decreased by 30% and 25%, respectively, in cells transfected with wild-type or C247S Txnip plasmid DNA/well compared to control-transfected cells (n=16; *p<0.001 vs control).