Skip to main content
. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Immunol. 2010 Mar 22;184(9):5000–5008. doi: 10.4049/jimmunol.0904064

Figure 3. Lyn A and Lyn B expressing mast cells differ in the extent of degranulation and each individual isoform fails to dampen cytokine production.

Figure 3

(A) Mast cell degranulation (as measured by release of hexosaminidase) is impaired in both Lyn A and Lyn B expressing cells relative to wild type (WT) cells. However, Lyn A elicits a more robust degranulation response at all antigen (Ag) concentrations tested than Lyn B. PMA and calcium ionophore (A23187) elicit degranulation of similar magnitude from the indicated genotypes. Data shown is a compilation of four experiments from individual cultures. More than ten experiments were performed. (C) Cytokine production (as measured by secretion into the medium) is hyperresponsive to FcεRI stimulation (4 hrs) in all genotypes (Lyn A, Lyn B, LacZ, and lyn−/−) relative to WT cells. The amount produced is normalized to 106 cells. Data shown (mean ± SD) is compiled from 3 individual experiments.