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. 2010 Sep 1;1:126–135. doi: 10.7150/jca.1.126

Figure 1.

Figure 1

Induction of transgene by doxycycline (Dox) in breast cancer cells stably transduced with a retroviral-mediated Tet-on system. 4T1 (A and B) and JygMC(A) (C and D) cells stably infected with Lenti-Tet-on GFP were treated with 1 μg/ml doxycycline (Dox, a tetracycline analogue) for 72 h (A-D). The cells were then observed under a fluorescent microscope for transgene GFP expression. The same image of the cells (4T1, A and B; JygMC(A), C and D, respectively) at both fluorescent (A and C) and light-contrast (B and D) images are shown. E and F. Immunohistochemcal (IHC) staining for p16 protein on MDA-MB-231 cells stably infected with Lenti-Tet-on p16 (MDA/Tet-on p16). MDA/Tet-on p16 cells were treated either without (E) or with (F) 1 μg/ml Dox for 72 h. The cells (E and F) were then IHC stained by primary anti-p16 antibody. The dark brown color indicates p16 protein (F).