Figure 5.

Hypoxia induced VEGF promoter transactivation and its inhibition by YC-1 involve HRE, the HIF-1α binding region of the promoter. MDA-MB-231 cells were transfected with pMAP11WT and pMAP11mut, followed by either normoxic or hypoxic conditions, and luciferase activity analysis as described in M & M section. A serial concentration of YC-1, a HIF-1α inhibitor, was added in the medium of cell samples under hypoxia in order to inhibit the hypoxia-induced HIF-1α transactivation activity. The normalized data is presented. The results represent the data from at least two independent experiments, each performed in duplicate.