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Hypoxia increased cell migration/motility in breast cancer cells. The breast cancer cells 4T1/Tet-on GFP cells were incubated with or without 1 μg/ml Dox for 72 h, followed by 24 h in hypoxic or normoxia incubation prior to be used for cell migration assay as described in M & M section. The results represent the data from at least two independent experiments, each performed in triplicate.
