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. 2010 Oct 1;1:141–149. doi: 10.7150/jca.1.141

Figure 4.

Figure 4

Inhibition of CCA cell proliferation by SS1P. Cancer cells (10,000 per well) were incubated with various concentrations of SS1P. Cell proliferation was measured by a WST assay as described in Materials and Methods. Each point represents the mean of triplicate experiments and the error bars indicate the standard deviation. The dashed line indicates 50% inhibition of cell viability, which is halfway between the level of viability in the absence of toxin and that in the presence of 10 μg/ml of cycloheximide. Each cell line and its IC50 (ng/mL): HuCCT1 (>1000 ng/ml for both SS1P and LMB2), HuH-28 (>1000 ng/ml for both SS1P and LMB2), KMBC (6.8 ng/ml for SS1P; 440.5 ng/ml for LMB2), KMCH (10.7 ng/ml for SS1P; >1000 ng/ml for LMB2), Mz-ChA-1 (9.1 ng/ml for SS1P; >1000 ng/ml for LMB2) and OZ (0.5 ng/ml for SS1P; >1000 ng/ml for LMB2). LMB2, a PE immunotoxin control specific for CD25.