Skip to main content
. Author manuscript; available in PMC: 2011 Oct 21.
Published in final edited form as: Org Biomol Chem. 2010 Aug 20;8(20):4601–4606. doi: 10.1039/c0ob00322k

Fig. 3.

Fig. 3

Utilization of the new reagents in a triplicate robotic screen of the LOPAC1280 library. (A) Excellent screening assay performance as evidenced by the consistently high Z' factor and the unchanging IC50 for a control inhibitor SCH-202676. (B) Switching to the new reagents did not change the assay's sensitivity to inhibitors as evidenced by the nearly identical dose responses obtained for two inhibitors of different potency, PD 404,182 (average IC50 3.2 μM) and calmidazolium chloride (average IC50 20 μM) when reagents 1 and 2 (PD 404,182, ○; calmidazolium chloride, □) or 13 and 20 (PD 404,12: ◆, ●, and *; calidazolium chloride: ■, ▲, and ▼) were used.