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NRG1 expression in the phrenic motor nucleus.
Maximum intensity projection of three confocal slices showing a longitudinal section at ~C3–4 (orientation: rostral, top; medial, left). Phrenic motoneurons (PhrMn) were retrogradely labeled by cholera toxin B (CTB; red) injected into the diaphragm muscle (see Methods). NRG1 (green) was widely distributed on PhrMn and other large neurons in the cervical ventral horn. Bar: 100 μm.
