Localization of NRG1 immunoreactivity at cholinergic synapses.
A. Maximum intensity projection of three consecutive confocal slices showing a retrogradely labeled PhrMn (blue), synapses labeled by NRG1 (green), and the vesicular acetylcholine transporter VAChT (red). Areas of co-localization that appear yellow designate the intersection between the VAChT- and NRG1-immunoreactivity. Bar: 20 μm.
B. Orthogonal projections of a single synapse (identified by an asterisk in A) showing co-localization of NRG1 and VAChT in 3D. NRG1 and VAChT partially intersect and they are clearly present at the same anatomical structure.