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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: J Comp Neurol. 2010 Oct 15;518(20):4213–4225. doi: 10.1002/cne.22449

Figure 7.

Figure 7

Localization of NRG1 immunoreactivity at cholinergic synapses.

A. Maximum intensity projection of three consecutive confocal slices showing a retrogradely labeled PhrMn (blue), synapses labeled by NRG1 (green), and the vesicular acetylcholine transporter VAChT (red). Areas of co-localization that appear yellow designate the intersection between the VAChT- and NRG1-immunoreactivity. Bar: 20 μm.

B. Orthogonal projections of a single synapse (identified by an asterisk in A) showing co-localization of NRG1 and VAChT in 3D. NRG1 and VAChT partially intersect and they are clearly present at the same anatomical structure.