Table 1.
List of primary antibodies
| Antigen | Immunogen | Manufacturer/Species | Dilution used |
|---|---|---|---|
| Cholera Toxin B | Highly purified B-subunit isolated from Vibrio cholerae Inaba 569B | List Biologicals (Campbell, CA) Goat polyclonal, Lot# 7023A5 |
1:500 |
| NRG-1 α/β1/2 | Peptide at C-terminus of the human NRG-1 isoform HRG-α (a.a. 620–640) | Santa Cruz Biotechnologies (Santa Cruz, CA) Rabbit polyclonal, sc-348, Lot# G1808 |
1:400 |
| Synaptophysin | Vesicular fraction of bovine brain | Chemicon/Millipore (Billerica, MA) Mouse monoclonal MAB 5258, clone SY 38 Lot# LV1414366 |
1:400 |
| VAChT | Peptide at the N-terminus of human VAChT (a.a. 1–33) | Santa Cruz Biotechnologies (Santa Cruz, CA) Goat polyclonal, sc-7717, Lot# D0307 |
1:100 |
| VGLUT1 | Strep-Tag fusion protein of rat VGLUT 1 (a.a. 456 – 560). | Synaptic Systems (Germany) Mouse monoclonal, clone 317D5, Lot# LV1417804 |
1:400 |
| VGLUT2 | Recombinant protein from rat VGLUT2 | Millipore (Billerica, MA) Mouse monoclonal MAB5504, Lot# LV11616024 |
1:400 |
NRG: neuregulin; VAChT: Vesicular Acetylcholine Transporter, VGLUT: Vesicular Glutamate Transporter